Human vascular smooth muscle cells both express and respond to heparin-binding growth factor I (endothelial cell growth factor)

Journal Article · Thu Oct 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.20.7124· OSTI ID:7026453

Winkles, J A; Friesel, R; Burgess, W H; Howk, R; Mehlman, T; Weinstein, R; Maciag, T

The control of vascular endothelial and muscle cell proliferation is important in such processes as tumor angiogenesis, wound healing, and the pathogenesis of atherosclerosis. Class I heparin-binding growth factor (HBGF-I) is a potent mitogen and chemoattractant for human endothelial cells in vitro and will induce angiogenesis in vivo. RNA gel blot hybridization experiments demonstrate that cultured human vascular smooth muscle cells, but not human umbilical cells also synthesize an HBGF-I mRNA. Smooth muscle cells also synthesize an HBGF-I-like polypeptide since (i) extract prepared from smooth muscle cells will compete with /sup 125/I-labeled HBGF-I for binding to the HBGF-I cell surface receptor, and (ii) the competing ligand is eluted from heparin-Sepharose affinity resin at a NaCl concentration similar to that required by purified bovine brain HBGF-I and stimulates endothelial cell proliferation in vitro. Furthermore, like endothelial cells, smooth muscle cells possess cell-surface-associated HBGF-I receptors and respond to HBGF-I as a mitogen. These results indicate the potential for an additional autocrine component of vascular smooth muscle cell growth control and establish a vessel wall source of HBGF-I for endothelial cell division in vivo.

• Research Organization: Meloy Labs., Inc., Rockville, MD (USA)
• OSTI ID: 7026453
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:20; ISSN PNASA
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
ANIMAL TISSUES
ANTICOAGULANTS
AORTA
ARTERIES
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
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CARDIOVASCULAR SYSTEM
CELL PROLIFERATION
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DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
GROWTH FACTORS
HEMATOLOGIC AGENTS
HEPARIN
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGANDS
LIGHT NUCLEI
MEMBRANE PROTEINS
MESSENGER-RNA
MITOGENS
MUCOPOLYSACCHARIDES
MUSCLES
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
RIBOSIDES
RNA
SACCHARIDES
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEINS