Identification of polycyclic aromatic hydrocarbon metabolites in mixtures using fluorescence line narrowing spectrometry

Journal Article · Wed May 01 04:00:00 EDT 1985 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00283a042· OSTI ID:5383808

Sanders, M J; Cooper, R S; Small, G J; Heisig, V; Jeffrey, A M

The direct identification of all six components in a laboratory mixture of polycyclic aromatic hydrocarbon (PAH) metabolites using fluorescence line narrowing (FLN) spectrometry is described. Metabolites are identified by comparison to standard spectra of the pure compounds. In addition, it is demonstrated that the sensitivity of the technique is adequate for the identification of PAH metabolite - DNA adducts at a level of approx.5 adducts per 10/sup 6/ bases. Typically, using cells in culture, DNA damage levels from PAH carcinogens are approx.1 adduct per 10/sup 5/ - 10/sup 6/ base pairs. The potential for the analysis of complex mixtures of DNA adducts is discussed. 22 references, 7 figures, 1 table.

• Research Organization: Iowa State Univ., Ames
• OSTI ID: 5383808
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 57:6; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104* -- Spectral Procedures-- (-1987)
560306 -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AROMATICS
CHEMICAL ANALYSIS
DATA
DNA ADDUCTS
EMISSION SPECTROSCOPY
EXPERIMENTAL DATA
FLUORESCENCE SPECTROSCOPY
HYDROCARBONS
INFORMATION
METABOLITES
NUMERICAL DATA
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
QUALITATIVE CHEMICAL ANALYSIS
SPECTROSCOPY