Identification of polycyclic aromatic hydrocarbon metabololites and DNA adducts in mixtures using fluorescence line narrowing spectrometry

Journal Article · Tue Apr 01 04:00:00 EST 1986 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00295a036· OSTI ID:5891310

Sanders, M J; Cooper, R S; Jankowiak, R; Small, G J; Heisig, V; Jeffrey, A M

Fluorescence line narrowing spectrometry is applied to five modifications of DNA (intact adducts) formed from diol epoxides of benzo(a)pyrene, chrysene, 5-methylchrysene, and benz(a)anthracene. The direct identification of all five adducts in a laboratory mixture is accomplished. In addition, a mixture of six corresponding metabolites plus the DNA adducts from benzo(a)pyrene and 5-methylchrysene is resolved. Each adduct can be distinguished from its corresponding tetrol metabolite. Utilization of an intensified diode array-optical multichannel analyzer provides detection of the adduct from benzo(a)pyrene with a S/N approx. 150 for a damage level of approx. 5 bases in 10/sup 6/. 25 references, 7 figures.

• Research Organization: Ames Lab., IA
• OSTI ID: 5891310
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 58:4; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104* -- Spectral Procedures-- (-1987)
400301 -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
ADDUCTS
AROMATICS
CARCINOGENS
CHEMICAL ANALYSIS
DATA
DISPERSIONS
DNA
EMISSION SPECTROSCOPY
EXPERIMENTAL DATA
FLUORESCENCE SPECTROSCOPY
HYDROCARBONS
INFORMATION
MIXTURES
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
QUALITATIVE CHEMICAL ANALYSIS
SPECTROSCOPY
STRUCTURAL CHEMICAL ANALYSIS