High resolution-sensitivity characterization of polycyclic aromatic hydrocarbon-DNA adducts using fluorescence line narrowing spectrometry
The application of fluorescence line narrowing spectrometry (FLNS) to the investigation of polar polycyclic aromatic hydrocarbon (PAH) metabolites and their corresponding DNA adducts is demonstrated. The selectivity is shown through the successful resolution of all components in separate mixtures of similar but distinct derivatives of benzo(a)pyrene, benz(a)anthracene, and chrysene. The separate mixtures were composed of six metabolites, five DNA adducts, each metabolite and its corresponding DNA adduct, and six metabolites and two DNA adducts. The broad applicability of FLNS is demonstrated through applications to the analysis of globin adducts, PAH metabolites in urine, and real samples, and to the investigation of carcinogenic metabolic pathways. 98 refs., 31 figs., 5 tabs.
- Research Organization:
- Ames Lab., IA (USA)
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 6414790
- Report Number(s):
- IS-T-1328; ON: DE89007611
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400102* -- Chemical & Spectral Procedures
ADDUCTS
ANTHRACENE
AROMATICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARCINOGENS
CHRYSENE
CONDENSED AROMATICS
DISPERSIONS
DNA ADDUCTS
ENERGY LEVELS
EXCITED STATES
FLUORESCENCE
GLOBIN
HYDROCARBONS
LASERS
LINE NARROWING
LUMINESCENCE
MATERIALS
MEASURING METHODS
METABOLITES
MIXTURES
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
PYRENE
RESOLUTION
SPECIFICITY
SPECTRA
SPECTROSCOPY
TIME RESOLUTION
TIMING PROPERTIES
URINE
VIBRATIONAL STATES
WASTES