Fluorescence line narrowing-nonphotochemical hole burning spectrometry. Femtomole detection and high selectivity for intact DNA-PAH adducts

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Chem. Res. Toxicol.; (United States)

DOI:https://doi.org/10.1021/tx00001a011· OSTI ID:6020681

Jankowiak, R; Cooper, R S; Zamzow, D; Small, G J; Doskocil, G; Jeffrey, A M

A new fluorescence line narrowing (FLN) apparatus is described and evaluated through experiments on intact DNA-PAH (polycyclic aromatic hydrocarbon) and globin-PAH adducts, as well as polar PAH metabolites. A detection limit of approx. 3 modified bases in 10/sup 8/ for a DNA adduct formed with a diol-epoxide of benzo(a)pyrene (BPDE-DNA) is reported for 20 ..mu..g of DNA at a spectral resolution of approx.8 cm/sup /minus/1/. The methodology employed avoids or minimizes spectral degradation and loss of sensitivity due to photooxidation and nonphotochemical hole burning (NPHB). A new double selection technique that employs both FLN and NPHB is described and found to lead to a significant improvement in selectivity over that obtained with conventional FLN.

• Research Organization: Iowa State Univ., Ames (USA)
• OSTI ID: 6020681
• Journal Information: Chem. Res. Toxicol.; (United States), Journal Name: Chem. Res. Toxicol.; (United States) Vol. 1:1; ISSN CRTOE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AROMATICS
BENZOPYRENE
CONDENSED AROMATICS
DNA ADDUCTS
FLUORESCENCE
GLOBIN
HYDROCARBONS
LUMINESCENCE
MEASURING METHODS
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
PROTEINS
SPECTRA