Relationship between radioactive microsphere and hydrogen gas (H/sub 2/) clearance estimates of pancreatic blood flow

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5383059

Kvietys, P R; Premen, A J; Granger, D N

The H/sub 2/ clearance method of measuring blood flow has been successfully applied to the stomach and intestines. The aim of the present study was to assess the validity of the H/sub 2/ clearance technique to measure pancreatic blood flow. Anesthetized Sprague-Dawley rats were instrumented to measure pancreatic blood flow with the H/sub 2/ clearance and microsphere methods. The pancreas was gently exposed via a midline incision and a platinum contact electrode was lightly applied to the tissue surface. Microspheres (15 ..mu..m in diameter and labelled with either /sup 46/Sc or /sup 85/Sr) were administered into the left ventricle via a cannula in the carotid artery. Estimates of pancreatic blood flow were obtained under control (resting) conditions and during administration of norepinephrine or isoproterenol. A direct linear relationship between H/sub 2/ clearance and microsphere measurements of pancreatic blood flow (r = 0.69) was obtained. It is concluded that the H/sub 2/ clearance technique maybe a useful method for obtained multiple estimates of pancreatic blood flow.

• Research Organization: Univ. of South Alabama, Mobile
• OSTI ID: 5383059
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD FLOW
BLOOD-PLASMA CLEARANCE
BODY
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
HOURS LIVING RADIOISOTOPES
HYDROGEN
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICROSPHERES
NONMETALS
NUCLEI
ODD-ODD NUCLEI
ORGANS
PANCREAS
RADIOISOTOPES
RATS
RODENTS
SCANDIUM 46
SCANDIUM ISOTOPES
SECONDS LIVING RADIOISOTOPES
STRONTIUM 85
STRONTIUM ISOTOPES
TRACER TECHNIQUES
VERTEBRATES