Glutathione peroxidase mRNA levels in selenium-deficient, selenium-adequate and high-selenium rats

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5372671

Sunde, R A; Schwartz, J K; Johnson, A W; Foley, N E ^[1]

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1. Univ. of Missouri, Columbia (United States)

Glutathione peroxidase (GPX) is an indicator of Se status, but the regulation of GPX is not understood. Se deficiency decreases GPX activity and mRNA levels in rat liver, but the effect of Se status on GPX mRNA levels in other tissues has not been investigated. To study this regulation, male weanling rats were fed Se-deficient, Se-adequate or high-Se diets. At 35 d, liver, kidney, heart, lung, muscle and testes GPX activities were assayed. mRNA levels were determined by northern blotting using a {sup 32}P-labeled 0.7 kb EcoRI fragment of cloned GPX. In Se-adequate rats, GPX enzyme activity was highest in liver and missing in testis; the ranking was L{much gt}K{gt}Lg{ge}H{gt}M{much gt}T. GPX mRNA level was also highest in liver and missing in testis; the ranking was L{much gt}K{gt}H{gt}Lg{ge}M{much gt}T. Se deficiency decreased the GPX mRNA level dramatically in liver, kidney and heart, and reduced it in lung and muscle, but high Se did not raise GPX mRNA levels above Se-adequate levels. These results show that GPX activity differences are due to different levels of GPX mRNA. The reduction of GPX mRNA levels by Se deficiency demonstrates that low Se status down-regulates GPX mRNA in other tissues as well as liver; high-Se status does not up-regulate GPX mRNA above Se-adequate levels. The tissue-to-tissue variation in GPX mRNA level indicates that other factors in addition to Se status regulate GPX expression.

• OSTI ID: 5372671
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DIET
DISTRIBUTION
DRUGS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GLUTATHIONE
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MESSENGER-RNA
METABOLISM
NUCLEI
NUCLEIC ACIDS
NUTRITIONAL DEFICIENCY
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
PEROXIDASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RATS
RNA
RODENTS
SELENIUM
SEMIMETALS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES