Sites of amyloid SAA mRNA production

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5322677

Meek, R L; Benditt, E P

To investigate possible extrahepatic sites of SAA production, male BALB/c mice were given a single 0.5 ml injection of either 10% casein or lipopolysaccharide (LPS; 100 mg/ml). Twenty hours after injection, RNA was extracted from liver, kidney, adrenal, testis, brain, spleen, skeletal muscle, heart, lung and small intestine. Northern blots of total RNA were hybridized with nick-translated /sup 32/P-labeled cDNA probes (length approximately 150 base pairs) corresponding to an homologous region of the three known SAA genes. Both casein and LPS elevated the mRNA in liver to about 200-fold above control levels; mRNA was elevated in adrenals from O to approximately 2% of liver. mRNA in some other tissues responded only to LPS injection: levels in kidney reached 15% of liver; pituitary, testis and brain reached 0.02 to 0.5% of liver; no apoSAA mRNA was detected in heart, skeletal muscle, lung, spleen or small intestine. Thus, some organs other than liver appear to have operational genes for apoSAA. The expression of apoSAA genes in different tissues is shared with other apoproteins; it remains to be seen whether all three or only selected genes are transcribed and translated in different tissues.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 5322677
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ANIMALS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
BRAIN
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
CASEIN
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DNA
ENDOCRINE GLANDS
GASTROINTESTINAL TRACT
GENES
GLANDS
GONADS
HEART
INTESTINES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
LIPIDS
LIPOPOLYSACCHARIDES
LIPOPROTEINS
LIVER
LUNGS
MALE GENITALS
MAMMALS
MESSENGER-RNA
MICE
MUSCLES
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RESPIRATORY SYSTEM
RNA
RODENTS
SACCHARIDES
SMALL INTESTINE
SPLEEN
SYNTHESIS
TESTES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES