Measurement of 3-0-methyldopamine in urine and plasma by a rapid and specific radioimmunoassay. [/sup 3/H tracer technique]
Journal Article
·
· J. Nucl. Med.; (United States)
OSTI ID:5372289
Antiserum against 3-0-methyldopamine (MD) was produced in rabbits immunized with MD hapten conjugated to hemocyanin. The antiserum was used to develop a radioimmunossay (RIA) for MD. As little as 0.5 ng of MD in 0.1 ml can be detected. The major catecholamines and the phenolic aromatic amines (dopamine, norepinephrine, epinephrine, octopamine, and tyramine) and their metabolites (normetanephrine, metanephrine, homovanillic acid and 4-hydroxy-3methxymandelic acid) did not bind significantly to the antibody. The RIA of MD was used to assay the endogenous level of MD in urine and plasma of hospitalized children. In children (7 mo to 13 yr), average concentration of MD in plasma was found to be 0.47 +- 0.11 ng/ml, and in urine 0.15 +- 0.05 ..mu..g/mg of creatinine (45.0 +- 16.3 ..mu..g/24 hr). In children with neuroblastoma, there was a 3- to 10-fold increase in urinary excretion and plasma level of 3-0-methyldopamine. In adults, the average urine and plasma levels were found to be 87.4 +- 3.4 ..mu..g/24 hr and 0.6 +- 0.2 ng/ml. The diagnostic applicability of the RIA of MD is discussed.
- Research Organization:
- Emory Univ., Atlanta
- OSTI ID:
- 5372289
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 18:10; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD PLASMA
BODY FLUIDS
DOPAMINE
DRUGS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
IMMUNE SERUMS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM
URINE
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD PLASMA
BODY FLUIDS
DOPAMINE
DRUGS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
IMMUNE SERUMS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM
URINE
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES