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Radioimmunoassay of metanephrine and normetanephrine for diagnosis of pheochromocytoma

Journal Article · · Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:6850155
; ; ; ; ;
Sensitive and specific radioimmunoassays of metanephrine and normetanephrine were developed by use of /sup 125/I-labeled synephrine and specific metanephrine antibody, and /sup 125/I-labeled octopamine and specific normetanephrine antibody. Specific antibody for both metanephrine and normetanephrine was raised in rabbits by immunization with bovine serum albumin conjugated with the corresponding hapten, prepared by the method of Grota and Brown (Endocrinology 1976;98:615). The detection limits of the metanephrine and the normetanephrine radioimmunoassays were 2 and 6 pg/tube, respectively. Mean plasma metanephrine and normetanephrine values for 24 normal subjects were 62 (SD 14) and 100 (SD 40) ng/L, respectively. Mean urinary metanephrine and normetanephrine values for 22 normal subjects were 154 (SD 74) and 217 (SD 109) micrograms/day. For 14 pheochromocytoma patients, plasma metanephrine and normetanephrine values ranged from 29 to 683 and from 28 to 7850 ng/L, and urinary metanephrine and normetanephrine values were 606 to 6630 and 296 to 4800 micrograms/day, respectively. The present methods are simple and suitable for routine tests or for mass screening for pheochromocytoma.
Research Organization:
Dainabot Co., Chiba, Japan
OSTI ID:
6850155
Journal Information:
Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 10; ISSN CLCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
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NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
STEROID HORMONES
TRACER TECHNIQUES
USES