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Development of an enzyme-radioimmunoassay for the measurement of dopamine in human plasma and urine

Journal Article · · J. Nucl. Med.; (United States)
OSTI ID:6393519
; ; ; ;
An enzyme-radioimmunoassay for the measurement of dopamine is described. It is based on the incubation of plasma or urine in the presence of catechol-0-methyltransferase and S-adenosylmethionine. The 0-methylated dopamine metabolite formed (3-0-methyldopamine) was characterized by radioimmunoassay. As little as 0.5 ng of dopamine can be detected. The assay was found to be specific, since no cross-reactivity was noted for several compounds related to dopamine. The enzyme-radioimmunoassay of dopamine was used to determine the concentrations of dopamine in urine and plasma of normal volunteers. In this group, urinary dopamine averaged 182.1 +- 2.2 ..mu..g/24 hr, and the plasma concentration 0.211 +- 0.052 ng/ml. However, in children wPth neuroblastoma, there was a several-fold increase over controls in the average urinary and plasma levels of dopamine (8,500 ..mu../24 hr and 2.3 ng/ml). The assay was also used to monitor blood levels of dopamine following the administration of L-dopa and dopamine to patients with cardiomyopathy.
Research Organization:
Emory Univ., Atlanta
OSTI ID:
6393519
Journal Information:
J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 19:11; ISSN JNMEA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
ANIMALS
AROMATICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD PLASMA
BODY FLUIDS
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
DOPAMINE
DRUGS
ENZYME ACTIVITY
HYDROXY COMPOUNDS
IMMUNE SERUMS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MAN
METABOLIC DISEASES
ORGANIC COMPOUNDS
PATIENTS
PHENOLS
POLYPHENOLS
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
SPECIFICITY
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
USES
VERTEBRATES
WASTES