Urine analysis for detection of cadmium-induced renal changes, with special reference to b/sub 2/-microglobulin

Journal Article · Wed Jun 01 04:00:00 EDT 1977 · Environ. Res.; (United States)

OSTI ID:5369368

Shiroishi, K ^[1]; Kjellstrom, T; Kubota, K; Evrin, P E; Anayama, M; Vesterberg, O; Shimada, T; Piscator, M; Iwata, T; Nishino, H

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1. Toyama Inst. of Health, Japan

An analysis of total protein, ..beta../sub 2/-microglobulin, glucose, and cadmium was performed on urine samples from people with Itai-itai disease and glomerular kidney disease, as well as on samples from a reference group. Blind analyses of creatinine, protein, and cadmium performed in Japanese and Swedish laboratories correlated well, but there was a tendency toward systematic differences between the laboratories' analyses of creatinine and protein. Within the range of the samples analyzed the difference was never larger than 30%. The use of three different methods of electrophoresis of urine proteins verified that the proteinuria in Itai-itai disease is tubular. On an average, urinary ..beta../sub 2/-microglobulin excretion among Itai-itai disease patients was 100 to 300 times higher than among the reference group, whereas total protein excretion was only 7 to 17 times higher. In a group of women with different degrees of cadmium exposure urinary excretion of proteins was evaluated with qualitative determination of protein, electrophoresis, and radioimmunoassay of ..beta../sub 2/-microglobulin. At slightly elevated ..beta../sub 2/-microglobulin excretion the first two methods gave negative results and it was concluded that radioimmunoassay of ..beta../sub 2/-microglobulin in urine is a sensitive indicator of cadmium-induced proteinuria.

• OSTI ID: 5369368
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 13:3; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMALS
ASIA
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CADMIUM
CARBOHYDRATES
CLEARANCE
ELECTROPHORESIS
ELEMENTS
EUROPE
EXCRETION
GLOBULINS
GLOBULINS-BETA
GLUCOSE
HEXOSES
ISOTOPE APPLICATIONS
JAPAN
KIDNEYS
MAMMALS
MAN
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
SACCHARIDES
SCANDINAVIA
SWEDEN
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES