Determination of carbonic anhydrase C and. beta. /sub 2/-microglobulin by radioimmunoassay in urine of heavy-metal-exposed subjects and patients with renal tubular acidosis

Journal Article · Mon Oct 01 04:00:00 EDT 1979 · Environ. Res.; (United States)

OSTI ID:5387891

Taniguchi, N ^[1]; Tanaka, M; Kishihara, C; Ohno, H; Kondo, T; Matsuda, I; Fujino, T; Harada, M

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1. Hokkaido Univ., Sapporo, Japan

Carbonic anhydrase C (CA-C) was measured by the radioimmunoassay in urine specimens from normal individuals, residents in mercury-polluted area, cadmium-exposed workers, lead-exposed workers, and patients with primary or secondary renal tubular acidosis. None of the urine in the normal subjects demonstrated CA-C levels above 40 ..mu..g/g creatinine. Some of the cadmium-exposed workers, residents in mercury-polluted area, and patients with renal tubular acidosis excreted large amounts of CA-C that were 10-250 times higher than the normal. Urinary ..beta../sub 2/-microglobulin (BMG) was also determined in relation to the CA-C levels. Most of the subjects exposed to heavy metals had high levels of urine CA-C or BMG or both. Some individuals had high levels of urine CA-C although BMG levels were within normal values. These findings seem to suggest that the mechanisms of urinary excretion of these two proteins were different and the quantitative determination of urinary CA-C by radioimmunoassay appears to be also a useful and sensitive test for detecting the renal tubular disorders in environmental exposure of heavy metals or those in renal tubular acidosis.

• OSTI ID: 5387891
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 20:1; ISSN ENVRA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
99 GENERAL AND MISCELLANEOUS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
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CARBONIC ANHYDRASE
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KIDNEYS
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MATERIALS
MEDICINE
MERCURY
METALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PERSONNEL
PROTEINS
QUANTITY RATIO
RADIOASSAY
RADIOIMMUNOASSAY
REACTION KINETICS
TOXICITY
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UROGENITAL SYSTEM DISEASES
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