Determination of carbonic anhydrase C and. beta. /sub 2/-microglobulin by radioimmunoassay in urine of heavy-metal-exposed subjects and patients with renal tubular acidosis

Journal Article · Mon Oct 01 00:00:00 EDT 1979 · Environ. Res.; (United States)

OSTI ID:5387891

Taniguchi, N ^[1]; Tanaka, M; Kishihara, C; Ohno, H; Kondo, T; Matsuda, I; Fujino, T; Harada, M

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1. Hokkaido Univ., Sapporo, Japan

Carbonic anhydrase C (CA-C) was measured by the radioimmunoassay in urine specimens from normal individuals, residents in mercury-polluted area, cadmium-exposed workers, lead-exposed workers, and patients with primary or secondary renal tubular acidosis. None of the urine in the normal subjects demonstrated CA-C levels above 40 ..mu..g/g creatinine. Some of the cadmium-exposed workers, residents in mercury-polluted area, and patients with renal tubular acidosis excreted large amounts of CA-C that were 10-250 times higher than the normal. Urinary ..beta../sub 2/-microglobulin (BMG) was also determined in relation to the CA-C levels. Most of the subjects exposed to heavy metals had high levels of urine CA-C or BMG or both. Some individuals had high levels of urine CA-C although BMG levels were within normal values. These findings seem to suggest that the mechanisms of urinary excretion of these two proteins were different and the quantitative determination of urinary CA-C by radioimmunoassay appears to be also a useful and sensitive test for detecting the renal tubular disorders in environmental exposure of heavy metals or those in renal tubular acidosis.

• OSTI ID: 5387891
• Journal Information: Environ. Res.; (United States), Vol. 20:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
CADMIUM
TOXICITY
CARBONIC ANHYDRASE
BIOCHEMICAL REACTION KINETICS
GLOBULINS-BETA
LEAD
MERCURY
PATIENTS
EXCRETION
PERSONNEL
EXPERIMENTAL DATA
INDUSTRIAL MEDICINE
ISOLATED VALUES
QUANTITY RATIO
RADIOIMMUNOASSAY
TUBULES
URINE
UROGENITAL SYSTEM DISEASES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CLEARANCE
DATA
DATA FORMS
DISEASES
ELEMENTS
ENZYMES
GLOBULINS
INFORMATION
ISOTOPE APPLICATIONS
KIDNEYS
KINETICS
LYASES
MATERIALS
MEDICINE
METALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
REACTION KINETICS
TRACER TECHNIQUES
WASTES
560306* - Chemicals Metabolism & Toxicology- Man- (-1987)
551000 - Physiological Systems
552000 - Public Health