Depletion of somatostatin-like immunoreactivity in the rat central nervous system by cysteamine
Selective neurotoxins have been of value in providing a means for specifically interfering with the actions of endogenous neurotransmitter candidates. Others have shown cysteamine (CSH) to deplete the gastrointestinal tract and hypothalamus of rats of immunoreactive somatostatin, suggesting a toxic action of that compound directed against somatostatin-containing cells. The present study further defines the actions of cysteamine on somatostatin in the central nervous system. (CNS). Cysteamine hydrochloride administered subcutaneously results in a depletion of somatostatin-like immunoreactivity (SLI) in the retina, brain, and cervical spinal cord of rats. The effect is demonstrable at doses of 30 mg/kg of body weight and above, occurs within 2 to 4 hr of a single injection of the drug, and is largely reversible within 1 week. The mean depletion of SLI observed within the CNS varies from 38% in cerebral cortex to 65% in cervical spinal cord 24 hr following administration of CSH, 300 mg/kg of body weight, s.c. By gel permeation chromatography, all molecular weight forms of SLI are affected, with the largest reductions in those forms that co-chromatograph with synthetic somatostatin-14 and somatostatin-28. These results indicate that CSH has a generalized, rapid, and largely reversible effect in depleting SLI from the rat CNS.
- OSTI ID:
- 5369268
- Journal Information:
- J. Neurosci. Res.; (United States), Vol. 2:2
- Country of Publication:
- United States
- Language:
- English
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CENTRAL NERVOUS SYSTEM
BIOLOGICAL MODELS
MEA
DOSE-RESPONSE RELATIONSHIPS
IMMUNE REACTIONS
NEUROLOGY
RATS
AMINES
ANIMALS
DRUGS
MAMMALS
MEDICINE
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOPROTECTIVE SUBSTANCES
RODENTS
THIOLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
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