Correlated effects of cigarette smoke components on alveolar macrophage adenosine triphosphatase activity and phagocytosis

Journal Article · Wed Jun 01 04:00:00 EDT 1977 · Am. Rev. Respir. Dis.; (United States)

OSTI ID:5361322

Low, E S; Low, R B; Green, G M

An initial examination was made of the hypothesis that one action of cigarette smoke components on pulmonary alveolar macrophage function involves the inhibition of contractile protein adenosine triphosphatase activity. Pulmonary alveolar macrophage calcium-dependent adenosine triphosphatase activity, magnesium-dependent adenosine triphosphatase activity, sodium-potassium-dependent adenosine triphosphatase activity, phagocytosis, and cell adhesiveness were measured in the presence of cigarette smoke, acrolein, ouabain, and ethacrynic acid. Calcium-dependent adenosine triphosphatase activity, magnesium-dependent adenosine triphosphatase activity, phagocytosis, and adhesiveness were inhibited by smoke and ethacrynic acid, but not by ouabain. Acrolein, a component of smoke, inhibited phagocytosis, adhesiveness, and calcium-dependent adenosine triphosphatase activity, indicating that another component of smoke must be effective at inhibiting magnesium-dependent adenosine triphosphatase activity. Sodium-potassium-dependent adenosine triphosphatase activity was inhibited by ouabain and ethacrynic acid, but not by smoke or acrolein. Finally, sulfhydryl reagents at least partially protected the macrophages against the inhibitory actions of each of the agents. The results are in accord with recently obtained experimental evidence that calcium-dependent adenosine triphosphatase and, perhaps, magnesium-dependent adenosine triphosphatase play a role in phagocytosis. The data also suggest that smoke components affect a number of macrophage activities, including adhesion and phagocytosis, by altering the cell's contractile apparatus.

• OSTI ID: 5361322
• Journal Information: Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 115:6; ISSN ARRDA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACROLEIN
AEROSOLS
ALDEHYDES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
COLLOIDS
COMPARATIVE EVALUATIONS
DISPERSIONS
DRUGS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESTERASES
GLYCOSIDES
HYDROLASES
LUNGS
MEASURING METHODS
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PHAGOCYTOSIS
PHOSPHATASES
RESIDUES
RESPIRATORY SYSTEM
SENSITIVITY
SMOKES
SOLS
STROPHANTHINS
TOBACCO SMOKES