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Separation of technetium hydroxyethylidene diphosphonate complexes by anion-exchange high performance liquid chromatography

Journal Article · · Anal. Chem.; (United States)
DOI:https://doi.org/10.1021/ac50057a025· OSTI ID:5346709
An anion-exchange high performance liquid chromatographic separation of technetium hydroxyethylidene diphosphonate (Tc-HEDP) complexes has been developed. Samples were prepared by the reduction of TcO/sub 4//sup -/ in the presence of HEDP with NaBH/sub 4/. Samples contained millimolar concentrations of /sup 99/Tc carrier and nanomolar concentrations of the ..gamma..-emitting /sup 99m/Tc isotope. Three modes of detection were used: (1) single wavelength at 405 nm, (2) uv-visible rapid scanning from 250 to 600 nm, and (3) single channel radiometric scintillation. The chromatograms illustrate that these radiopharmaceutical analogues consist of mixtures of at least seven Tc-containing species of unknown oxidation state, central metal configuration, and coordination environment. The distribution of components in these mixtures is dependent on reaction time and the presence of molecular oxygen. 9 figures, 1 table.
Research Organization:
Univ. of Cincinnati, OH
OSTI ID:
5346709
Journal Information:
Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 52:7; ISSN ANCHA
Country of Publication:
United States
Language:
English