Characterization of technetium hydroxyethylidene diphosphonate complexes prepared by sodium borohydride reduction

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5897176

Wilson, G M

The fundamental solution chemistry and biodistribution of technetium hydroxyethylidene diphosphonate complexes (Tc-HEDP), prepared by the sodium borohydride reduction of pertechnetate in the presence of the ligand, has been investigated. An anion-exchange high-performance liquid chromatography separation was developed that allowed the individual Tc-HEDP complexes to be isolated and studied. Complimentary HPLC detection schemes were employed, including fixed wavelength UV-visible spectrophotometric detection and/or radiometric detection of /sup 99m/Tc. Characteristic UV-visible spectra were obtained for many of the Tc-HEDP complexes by utilizing UV-visible photodiode array HPLC detection. The charge on twelve of the Tc-HEDP complexes was determined by anion-exchange HPLC, and from these results and size-exclusion chromatography data, a polynuclear hypothesis regarding the structure of the complexes has been developed. The biodistribution of six of the /sup 99m/Tc-HEDP complexes separated by anion-exchange HPLC was studied. An inverse structure-activity correlation was determined to exist between /sup 99m/Tc uptake in new (lesion) bone and the physical properties, anionic charge and partial molar volume, of the complexes. The uptake in the lesion bone was found to be inversely related to the charge and/or size of the complexes. The results were consistent with the polynuclear hypothesis previously suggested for the Tc-HEDP complexes. A new Tc-formate system was studied with the prospect that it might serve as a ligand-exchange precursor to the synthetic preparation and characterization of the Tc-HEDP complexes.

• Research Organization: Purdue Univ., Lafayette, IN (USA)
• OSTI ID: 5897176
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201* -- Chemical & Physicochemical Properties
400301 -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACID SALTS
CHEMICAL PREPARATION
CHROMATOGRAPHY
COMPLEXES
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DIENES
FORMATES
HOURS LIVING RADIOISOTOPES
HYDROCARBONS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
POLYENES
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
SEPARATION PROCESSES
SKELETON
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TECHNETIUM 99
TECHNETIUM COMPLEXES
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
UPTAKE
YEARS LIVING RADIOISOTOPES