Animal disease detection using a new rapid method for monitoring antigen-induced lymphocyte stimulation, October 1, 1978-September 30, 1979
Lymphocyte proliferation in response to bovine tubercular antigen was measured on blood samples from a herd naturally infected with TB. Determination of single-cell DNA content as a measure of cell proliferation was done by flow cytometry after staining with the DNA-specific stain, propidium iodide. Results of the field testing showed good agreement with other methods for TB detection. Significant progress on the development of a new method for TB detection was made. An increase in the rate of amino acid uptake can be measured within 14 h after treatments of lymphocytes from TB-positive animals with TB antigen. The enhancement does not occur after similar treatment of lymphocytes from TB-negative animals. Ways for optimizing the new test and investigations into other new diagnostic techniques are reported.
- Research Organization:
- Los Alamos Scientific Lab., NM (USA)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 5340332
- Report Number(s):
- LA-8227-PR
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
60 APPLIED LIFE SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CATTLE
CELL FLOW SYSTEMS
CONNECTIVE TISSUE CELLS
DISEASES
DOMESTIC ANIMALS
FLUORESCENCE
INFECTIOUS DISEASES
LEUKOCYTES
LUMINESCENCE
LYMPHOCYTES
MAMMALS
MATERIALS
RUMINANTS
SCREENING
SOMATIC CELLS
TUBERCULOSIS
VERTEBRATES