Flow microfluorometric quantitation of the blastogenic response of lymphocytes

Journal Article · Thu Jan 01 04:00:00 EST 1976 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/24.1.768372· OSTI ID:7306916

Cram, L S; Gomez, E R; Thoen, C O; Forslund, J C; Jett, J H

A method for quantitating the specific stimulation of peripheral lymphocytes has been developed using the techniques of flow microfluorometry. Peripheral bovine lymphocytes were collected and specifically stained for deoxyribonucleic acid (DNA) content using a low-salt propidium iodide procedure. Flow microfluorometry was used to determine, on the basis of DNA content, the percentage of cells in a population that was stimulated. Extremely uniform staining of the lymphocytes (coefficients of variation of less than 2 percent) provides a high resolution between proliferating and nonproliferating cells. The method provides a rapid, highly reproducible technique for determining the fraction of lymphocytes stimulated in response to tuberculin antigens based on an increase in cellular DNA content. Specific and nonspecific stimulation by a defined antigen can be measured and resolved.

• Research Organization: Los Alamos Scientific Lab., NM
• OSTI ID: 7306916
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 24:1; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DNA
LEUKOCYTES
LYMPHOCYTES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SOMATIC CELLS
STAINS