T lymphocyte-dependent B lymphocyte proliferative response to antigen. I Genetic restriction of the stimulation of B lymphocyte proliferation

Journal Article · Wed Apr 01 04:00:00 EST 1981 · J. Exp. Med.; (United States)

DOI:https://doi.org/10.1084/jem.153.4.871· OSTI ID:5674647

Tse, H Y; Mond, J J; Paul, W E

For the purpose of examining more closely the interaction between T and B lymphocytes, we have developed an in vitro T lymphocyte-dependent B lymphocyte proliferation assay. Proliferation of B lymphocytes in response to antigen was found to depend on the presence of primed T lymphocytes; the B lymphocytes could be derived from nonprimed animals. It appears that these B cells were nonspecifically recruited to proliferate. This nonspecific recruitment, however, was found to be Ir-gene restricted in that B lymphocytes from B10.S mice, which are genetic nonresponders to the polymer Glu60-Ala30-Tyr10 (GAT), could not be stimulated by GAT-primed (responder X nonresponder) F1 T cells. The apparent lack of antigen specificity in the face of Ir gene-restricted T-B interaction may have important implications in our understanding of the recognition unit(s) on T lymphocytes.

• OSTI ID: 5674647
• Journal Information: J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 153:4; ISSN JEMEA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIGENS
BIOASSAY
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
GENETICS
IMMUNITY
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MATERIALS
SOMATIC CELLS
STIMULATION