Application of cell analysis and sorting techniques to disease detection. Progress report, October 1, 1977--September 30, 1978

Technical Report · Tue May 01 00:00:00 EDT 1979

DOI:https://doi.org/10.2172/6252955· OSTI ID:6252955

Forslund, J C; Brake, E T; Cram, L S

Significant progress has been made on the development and characterization of a cell-mediated assay for bovine tuberculosis. Using a whole blood culture technique, lymphocyte proliferation in response to a purified protein derivative from Mycobacterium avium (PPD-A) or Mycobacterium bovis (PPD-B) was measured. The analysis was done by flow microfluorometry of fluorescently stained lymphocyte DNA. Samples from experimentally infected animals and field cases were evaluated using this procedure. Optimization of the protocol is reported and investigations into new diagnostic techniques are reported.

• Research Organization: Los Alamos Scientific Lab., NM (USA)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 6252955
• Report Number(s): LA-7791-PR
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BACTERIA
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
CYTOLOGICAL TECHNIQUES
DATA
DATA FORMS
DIAGNOSTIC TECHNIQUES
DISEASES
DNA
EXPERIMENTAL DATA
GRAPHS
INFECTIOUS DISEASES
INFORMATION
LEUKOCYTES
LYMPHOCYTES
MICROORGANISMS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
SOMATIC CELLS
TUBERCULOSIS