Effect of dietary fibers on cholic acid induced cell proliferation in the colonic epithelium of C57BL/6J mice
It has been postulated that high fat diets promote tumorigenesis by increasing the level of secondary bile acids in the colonic lumen. Dietary fibers are thought to be protective perhaps through their interaction with bile acids. In the present study, animals were fed diets containing either 0%, 5%, or 10% cellulose (C), pectin (P), or wheat bran (WB). The diets were formulated to contain either 0% (control) or 0.2% cholic acid (test). After two weeks of dietary treatment the animals were injected with (/sup 3/H)-thymidine and their colons were processed for autoradiography. The number of labeled cells (LC) in the colonic crypts was determined. Among the control diets, 10%P induced a two-fold increase in the LC. All the test groups had significantly higher LC than in their controls. However, the C group excited a higher LC than the P or WB groups (5.2 +/- 0.8 vs 3.9 +/- 0.8 or 3.9 +/- 0.6). These results were substantiated by metaphase arrest technique. The authors results show that nonfermentable fiber does not alleviate bile acid induced cell proliferative activity in the colon whereas fermentable fibers will counteract the promotional effect of a high fat diet.
- Research Organization:
- Univ. of Toronto, Ontario
- OSTI ID:
- 5332185
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZINES
BILE ACIDS
BIOLOGICAL EFFECTS
BLOOD SUBSTITUTES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARCINOGENESIS
CELL PROLIFERATION
CELLULOSE
CEREALS
CHOLIC ACID
CRYPT CELLS
DIET
DIGESTIVE SYSTEM
DRUGS
FIBERS
GASTROINTESTINAL TRACT
GRASS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTESTINES
LABELLED COMPOUNDS
LARGE INTESTINE
MAMMALS
MICE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PECTINS
PLANTS
POLYSACCHARIDES
PYRIMIDINES
RIBOSIDES
RODENTS
SACCHARIDES
SOMATIC CELLS
STEROIDS
STEROLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
WHEAT