Influence of diet or intrarectal bile acid injections on colon epithelial cell proliferation in rats previously injected with 1,2-dimethylhydrazine

Journal Article · Tue Mar 01 00:00:00 EST 1983 · J. Nutr.; (United States)

OSTI ID:5602879

Glauert, H P; Bennink, M R

The effects of varying colon bile acid concentrations on rat colon epithelial cell proliferation were studied. Bile acid concentrations were altered by intrarectally injecting either deoxycholic or lithocholic acid for 4 weeks or by increasing the dietary fat or fiber (wheat bran, agar, or carrageenan) intake for 4 weeks. 1,2-Dimethylhydrazine (DMH) was s.c. injected into half of the rats 1 week before treatments began. Colon epithelial cell proliferation was measured by (/sup 3/H)thymidine autoradiography of colon crypts. Rats injected with DMH had more DNA-synthesizing cells per crypt. Neither bile acid injection nor any of the diets altered the number of DNA-synthesizing cells per crypt. DMH injections, deoxycholic and lithocholic acid intrarectal injections, and dietary agar and wheat bran all increased the total number of cells per crypt. High fat diets and dietary carrageenan did not affect cell number. All diets containing fiber lowered total fecal bile acid concentrations, but increasing the fat content of the diet did not affect them. These results indicate that the bile acid injections and dietary agar and wheat bran induce a slight hyperplasia in the colon.

• Research Organization: Department of Food Science and Human Nutrition, Michigan State University, East Lansing
• OSTI ID: 5602879
• Journal Information: J. Nutr.; (United States), Vol. 113:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BILE
BIOLOGICAL EFFECTS
DIET
EPITHELIUM
AUTORADIOGRAPHY
CELL PROLIFERATION
DNA
HYDRAZINE
LARGE INTESTINE
RATS
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
INTESTINES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NITROGEN COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RIBOSIDES
RODENTS
TISSUES
VERTEBRATES
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