Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Isotope dilution measurement of copper absorption and excretion in rats fed different carbohydrates

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5321288
;
An isotope dilution method using /sup 67/Cu was developed to measure true absorption (A) and endogenous excretion (EE) of Cu in rats. Specific activity (SA) of injected /sup 67/Cu was least variable in 7 tissues on days 6-8 after injection. SA of feces compared to tissues was used to calculate EE and A. This method was used to study Cu metabolism in rats fed 5 ppm or 0.4 ppm Cu and diets containing fructose (FR), glucose (GL), sucrose (SU), or cornstarch (CS). In rats fed 5 ppm Cu, the A, EE, and balance (B) were greatest for CS animals. There were no differences in A, B, or EE between FR and SU rats (p > .05). Rats fed GL had B and A lower than other groups (p < .05) but EE was different only from CS rats. Liver and kidney Cu and ceruloplasmin levels were highest in CS rats; other groups did not differ significantly. For rats fed 0.4 ppm Cu, the A, B, and EE did not differ among groups fed different carbohydrates (p > .05). Liver Cu did not differ among groups fed 0.4 ppm Cu. Kidney Cu was higher ( p < .001) in CS rats than GL rats; other groups did not differ. B was significantly lower in rats on the 0.4 ppm Cu diet than on 5 ppm Cu for CS, FR, SU (p < .05). Rats fed the GL-low Cu diet had significantly higher A (p < .01) and unchanged B (p > .05) compared to rats fed GL and 5 ppm Cu. EE was significantly lower in all groups on 0.4 ppm Cu than 5 ppm Cu. EE was 1 ..mu..g Cu/d on the 0.4 ppm Cu diet.
Research Organization:
Dept. of Agriculture, Grand Forks, ND
OSTI ID:
5321288
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

Similar Records

Uptake of radiolabeled copper by the liver as affected by fructose or glucose
Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5450416
Effects of dietary carbohydrate on iron metabolism and cytochrome oxidase activity in copper-deficient rats
Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5381840
Consequences of copper (Cu) deficiency are not differentially influenced by fructose or starch in growing pigs fed a dried skim milk-based diet
Journal Article · Wed Feb 08 23:00:00 EST 1989 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA) · OSTI ID:7190437

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ALDEHYDES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
CARBOHYDRATES
CLEARANCE
COPPER
COPPER 67
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
DIET
DIGESTIVE SYSTEM
DISACCHARIDES
ELEMENTS
EXCRETION
FECES
FRUCTOSE
GLANDS
GLUCOSE
HEXOSES
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
KETONES
KIDNEYS
LIVER
MAMMALS
MATERIALS
METABOLISM
METALS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SACCHAROSE
TRACER TECHNIQUES
TRANSITION ELEMENTS
UPTAKE
VERTEBRATES
WASTES