Role of sulfhydryl groups in the functioning of DNA-dependent RNA polymerase
The chemical modification has been performed of the sulfhydryl groups of Escherichia coli DNA-dependent RNA polymerase with alkylating and mercury-containing compounds. It has been shown that iodoacetic acid and iodacetamide do not affect the activity of the enzyme, while N-ethylmaleimide and mercury-containing compounds inhibit RNA syntheses catalyzed by it. RNA polymerase modified with Hg/sup 2 +/ ions loses its capacity for binding with promoter-containing fragments of DNA. Furthermore, mercury ions also inhibit the RNA-elongation stage. A hypothesis has been put forward concerning the participation of the SH groups of the enzyme in the process of untwisting double-stranded DNA. It has been shown that the SH groups of the ..beta..- and ..beta..'-subunits of RNA polymerase participate in the binding of double-stranded fragments of DNA.
- Research Organization:
- M. M. Shemyakin Institute of Bioorganic Chemistry, Moscow (USSR)
- OSTI ID:
- 5280780
- Journal Information:
- Sov. J. Bioorg. Chem. (Engl. Transl.); (United States), Journal Name: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States) Vol. 11:4; ISSN SJBCD
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID
AMIDES
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BINDING ENERGY
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CYSTEINE
DAYS LIVING RADIOISOTOPES
DICARBOXYLIC ACIDS
DNA
ELONGATION
ENERGY
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESCHERICHIA COLI
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MALEIC ACID
MERCURY COMPOUNDS
MESSENGER-RNA
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MORPHOLOGICAL CHANGES
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADICALS
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RNA
RNA POLYMERASES
SULFHYDRYL RADICALS
SYNTHESIS
THIOLS
TRANSCRIPTION
TRANSFERASES
UTP