Effect of Escherichia coli DNA binding protein on the transcription of single-stranded phage M13 DNA by Escherichia coli RNA polymerase

Journal Article · Fri Sep 09 04:00:00 EDT 1977 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(77)91260-8· OSTI ID:5167626

Niyogi, S K; Ratrie, H III; Datta, A K

E. coli DNA binding protein strongly inhibits the transcription of single-stranded rather than double-stranded phage M13 DNA by E. coli RNA polymerase. This inhibition cannot be significantly overcome by increasing the concentration of RNA polymerase. Nor does the order of addition of binding protein affect its inhibitory property: inhibition is evident whether binding protein is added before or after the formation of the RNA polymerase--DNA complex. Inhibition is also observed if binding protein is added at various times after initiation of RNA synthesis. Maximal inhibition occurs at a binding protein-to-DNA ratio (w/w) of about 8:1. This corresponds to one binding protein molecule covering about 30 nucleotides, in good agreement with values obtained by physical measurements.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 5167626
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 78:1; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CHEMISTRY
DNA
ENZYMES
ESCHERICHIA COLI
INHIBITION
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
POLYMERASES
PROTEINS
RNA
SYNTHESIS
TRANSFER RNA