Role of Escherichia coli dnaG function in coliphage M13 DNA synthesis
Journal Article
·
· Eur. J. Biochem.; (United States)
Examination of the role of Escherichia coli dnaG function in different stages of M13 phage DNA synthesis by ultracentrifugal analysis of intracellular phage DNA in a thermosensitive dnaG mutant shows that: (a) the formation of parental double-strand replicative-form DNA (rfDNA) from the infecting virus is independent of dnaG function; (b) the synthesis of progeny rfDNA requires dnaG product; (c) after a pool of rfDNA is made up, dnaG function is not required for the progeny single-strand DNA (ssDNA) synthesis. The ssDNAs produced under nonpermissive condition are mostly circular and biologically functional.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 7231974
- Journal Information:
- Eur. J. Biochem.; (United States), Journal Name: Eur. J. Biochem.; (United States) Vol. 67; ISSN EJBCA
- Country of Publication:
- United States
- Language:
- English
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