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Replication of UV-irradiated single-stranded DNA by DNA polymerase III holoenzyme of Escherichia coli: evidence for bypass of pyrimidine photodimers

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
Replication of UV-irradiated circular single-stranded phage M13 DNA by Escherichia coli RNA polymerase (EC 2.7.7.6) and DNA polymerase III holoenzyme (EC 2.7.7.7) in the presence of single-stranded DNA binding protein yielded full-length as well as partially replicated products. A similar result was obtained with phage G4 DNA primed with E. coli DNA primase, and phage phi X174 DNA primed with a synthetic oligonucleotide. The fraction of full-length DNA was several orders of magnitude higher than predicted if pyrimidine photodimers were to constitute absolute blocks to DNA replication. Recent models have suggested that pyrimidine photodimers are absolute blocks to DNA replication and that SOS-induced proteins are required to allow their bypass. Our results demonstrate that, under in vitro replication conditions, E. coli DNA polymerase III holoenzyme can insert nucleotides opposite pyrimidine dimers to a significant extent, even in the absence of SOS-induced proteins.
OSTI ID:
5092115
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 13; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA POLYMERASES
DNA REPAIR
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEOTIDYLTRANSFERASES
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PYRIMIDINE DIMERS
RADIATIONS
RECOVERY
REPAIR
TRANSFERASES
ULTRAVIOLET RADIATION
VIRUSES