Selenotrisulfide inhibits initiation by RNA polymerase II but not elongation
Journal Article
·
· J. Inorg. Biochem.; (United States)
We previously reported that RNA polymerase II (purified from wheat germ) is inhibited by selenotrisulfides, the products of the reaction of selenite with sulfhydryl compounds. We have now found that the initiation stage of the reaction is inhibited by selenotrisulfide but the elongation stage of the reaction is not. The actual start of the RNA chain is not inhibited by the selenotrisulfide, but rather the formation of the enzyme-DNA binary complex. Selenotrisulfide has a similar differential effect on initiation and elongation by RNA polymerase II from HeLa cells; in contrast, with E. coli RNA polymerase, it inhibits elongation as well.
- Research Organization:
- Rutgers Univ., Newark, NJ (USA)
- OSTI ID:
- 5714374
- Journal Information:
- J. Inorg. Biochem.; (United States), Journal Name: J. Inorg. Biochem.; (United States) Vol. 35:3; ISSN JIBID
- Country of Publication:
- United States
- Language:
- English
Similar Records
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OSTI ID:5145203
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Journal Article
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMALS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CEREALS
CHALCOGENIDES
DRUGS
ELEMENTS
ENZYMES
ESCHERICHIA COLI
GLUTATHIONE
GRASS
HELA CELLS
INHIBITION
KINETICS
LILIOPSIDA
MAGNOLIOPHYTA
MAMMALS
MAN
MEA
MICROORGANISMS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RNA POLYMERASES
SELENIUM
SEMIMETALS
SULFIDES
SULFUR COMPOUNDS
THIOLS
TRANSCRIPTION
TRANSFERASES
VERTEBRATES
WHEAT
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMALS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CEREALS
CHALCOGENIDES
DRUGS
ELEMENTS
ENZYMES
ESCHERICHIA COLI
GLUTATHIONE
GRASS
HELA CELLS
INHIBITION
KINETICS
LILIOPSIDA
MAGNOLIOPHYTA
MAMMALS
MAN
MEA
MICROORGANISMS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RNA POLYMERASES
SELENIUM
SEMIMETALS
SULFIDES
SULFUR COMPOUNDS
THIOLS
TRANSCRIPTION
TRANSFERASES
VERTEBRATES
WHEAT