Inhibition of DNA and RNA polymerases by sodium selenite and sulfhydryl compounds
They have previously shown that sodium selenite has very little, if any, inhibitory effect on DNA and RNA polymerases. They have now found, however, that selenite does cause significant inhibition of DNA and RNA polymerases in the presence of sulfhydryl compounds such as mercaptoethanol and mercaptoethylamine (which themselves have no significant effect on the activity of the enzymes). They have found that there are differences between different enzymes in their sensitivity to inhibition by selenite in the presence of sulfhydryl compounds. Furthermore, there are differences between enzymes in the relative potencies of different sulfhydryl compounds in potentiating the inhibitory effect of selenite. The inhibitory species appears to be the selenotrisulfide adduct described previously by Ganther which forms from the reaction of one mole of selenite with two moles of sulfhydryl compound.
- Research Organization:
- Rutgers, The State Univ. of New Jersey, New Brunswick
- OSTI ID:
- 5145203
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
DNA POLYMERASES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
INHIBITION
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RNA POLYMERASES
SELENIUM
SEMIMETALS
SENSITIVITY
SYNERGISM
THIOLS
TRANSFERASES