Effect of cycloheximide on the inhibition of RNA synthesis and colony formation by selenite

Conference · Sun Mar 10 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5372144

Frenkel, G D; Yan, L ^[1]

---

1. Rutgers-the State Univ., Newark, NJ (United States)

Previous studies in this laboratory have shown that reaction with cellular sulfhydryl compounds is necessary for selenite to inhibit RNA synthesis and colony formation, most probably due to the requirement for the formation of selenotrisulfide derivatives. The inhibitory effects of selenite could be a direct effect of these derivatives, or could be mediated by induced selenoprotein(s). In order to determine whether cellular protein synthesis is necessary for selenite to inhibit RNA synthesis and colony formation, the authors have examined the effect of cycloheximide on this inhibition. Incubation of HeLa cells with cycloheximide resulted in 95% inhibition of protein synthesis (as measured by ({sup 3}H)leucine incorporation) but had no effect on RNA synthesis (as measured by ({sup 3}H)uridine incorporation) or on the ability of cells to form colonies. Incubation with cycloheximide prior to selenite exposure had very little, if any, effect on the selenite-induced inhibition of RNA synthesis and colony formation. These results indicate that protein synthesis is not essential for these cytotoxic effects of selenite.

• OSTI ID: 5372144
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN 0892-6638; ISSN FAJOE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AZINES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
COLONY FORMATION
CYCLOHEXIMIDE
DRUGS
ELEMENTS
FUNGICIDES
HELA CELLS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
LEUCINE
METABOLISM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PESTICIDES
PYRIMIDINES
RIBOSIDES
RNA
SELENIUM
SEMIMETALS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
URIDINE