Cytological and molecular studies of chromosomal radiosensitivity in Down Syndrome cells
Thesis/Dissertation
·
OSTI ID:5259583
Molecular, cellular and cytogenetic studies were conducted to determine if altered levels of poly(ADP-ribose) polymerase, a DNA repair-related enzyme, is responsible for the reported formation of excess X-ray induced chromosome aberrations in cells derived from Down Syndrome (DS) patients. Nonstimulated lymphocytes from normal and DS subjects were pretreated with 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase, for 30 minutes before exposure to X-rays and the levels of induced chromosome aberrations were determined in mitotic cells. DS lymphocytes exhibited significantly higher frequencies of chromosome aberrations in the presence of 3-aminobenzamide that normal lymphocytes. No difference was observed in the absence of 3-aminobenzamide. Additional studies were done using normal and DS lymphoblastoid cell lines which exhibited a similar response at the DNA level as the lymphocytes. Analysis of poly(ADP-ribose) polymerase activity based on incorporation of the substrate, NAD{sup +}, into acid insoluble materials, revealed that there was no significant difference in the ability to form poly (ADP-ribose) in the DS or normal cells. 3-aminobenzamide effectively inhibited poly(ADP-ribose) polymerase in both the normal and DS cells.
- Research Organization:
- Texas Univ., Galveston, TX (USA)
- OSTI ID:
- 5259583
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CONGENITAL MALFORMATIONS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DNA REPAIR
DOWNS SYNDROME
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HEREDITARY DISEASES
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MALFORMATIONS
MATERIALS
MOLECULAR BIOLOGY
MUTATION FREQUENCY
MUTATIONS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SOMATIC CELLS
TRANSFERASES
X RADIATION
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CONGENITAL MALFORMATIONS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DNA REPAIR
DOWNS SYNDROME
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HEREDITARY DISEASES
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MALFORMATIONS
MATERIALS
MOLECULAR BIOLOGY
MUTATION FREQUENCY
MUTATIONS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SOMATIC CELLS
TRANSFERASES
X RADIATION