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Acidic extracellular environment reduces the fixation of radiation damage

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576196· OSTI ID:5245607
Chinese hamster ovary cells in exponential growth were irradiated with ..gamma.. or X rays in an acidic or alkaline extracellular environment. Incubation in acid medium during or after irradiation reduced the degree of fixation of potentially lethal lesions. Fixation and repair of acid-modified damage occurred with a halftime of 9 to 10 min. The rate of repair of sublethal damage was unaffected by hydrogen ion concentration and progressed with a half-time of 30 min. An acid environment modified only survival of exponentially growing cells; unfed plateau-phase cultures were unaffected.
Research Organization:
Columbia Univ. Coll. of Physicians and Surgeons, New York, NY
DOE Contract Number:
AC02-78EV04733
OSTI ID:
5245607
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 97:1; ISSN RAREA
Country of Publication:
United States
Language:
English