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Effect of extreme hypoxia and glucose on the repair of potentially lethal and sublethal radiation damage by mammalian cells. [X Radiation]

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3574644· OSTI ID:5114243
; ;
Confluent monolayers of Chinese hamster ovary cells repaired potentially lethal x-ray damage under aerobic conditions but did not repair such damage under extremely hypoxic conditions. However, addition of extra glucose to the medium allowed some repair of potentially lethal damage by these hypoxic cells. Because of this effect of glucose, we repeated earlier experiments, which had shown that exponentially growing cells did not repair sublethal damage under extremely hypoxic conditions using both plateau-phase and exponentially growing cells. Two significant results were obtained under extremely hypoxic conditions, no repair of sublethal damage was observed, even at high glucose concentrations; under aerobic conditions, plateau-phase cells repaired sublethal damage more rapidly and more efficiently than did similarly treated exponentially growing cells. From these data we conclude that the oxygen and/or energy requirements are different for the repair of potentially lethal and sublethal damage.
Research Organization:
Victoria Hospital, London, Ontario
OSTI ID:
5114243
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 70:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL CELLS
ANIMALS
ANOXIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CRYOGENIC FLUIDS
ELECTROMAGNETIC RADIATION
ELEMENTS
FLUIDS
GLUCOSE
HAMSTERS
HEXOSES
IONIZING RADIATIONS
MAMMALS
MONOSACCHARIDES
NONMETALS
ORGANIC COMPOUNDS
OXYGEN
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SACCHARIDES
VERTEBRATES
X RADIATION