Cadmium-109 uptake by tumors derived from Balb C/3T3 cell lines with varying degrees of the transformed phenotype

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5244205

Morton, K; Alazraki, N; Winge, D; Lynch, R E

To determine if tumors are rich in metallothionein, the authors measured the vivo uptake of subcutaneously-injected carrier-free cadmium-109 in tumors and in normal tissues of Balb/C mice. The tumors were grown in the mice from cultured Balb/3T3 cells transformed by the Moloney murine sarcoma virus. Uptake of cadmium-109 per gram of tissue was greatest for liver, kidney, and spleen. However, tumor uptake of cadmium-109 was markedly greater than that in blood, skeletal muscle, bones, intestine or adipose tissue. B Sephadex G-75 chromatography, the radioactivity in tumor and in liver lysates eluted with cytochrome-C, a molecule similar in molecular weight to metal-lothionein. To determine if metallothionein levels are related to the degree of malignancy of tumors, cadmium-109 uptake in the tumors from the virally-transformed cells was compared to that in tumors from non-transformed Balb/3T3 cells and two derivative chemically transformed cell lines. There was strong correlation between the substrate-independent growth in soft agarose of the four cell lines, the rate of growth of the corresponding tumors, and the amount of cadmium-109 uptake in the tumors. The authors conclude that metallothionein levels may be elevated in tumors as a function of the degree of expression of the transformed phenotype.

• Research Organization: Virginia Medical Center, Salt Lake City, UT
• OSTI ID: 5244205
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CADMIUM
CADMIUM 109
CADMIUM ISOTOPES
CHROMATOGRAPHY
CONNECTIVE TISSUE
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
GAS CHROMATOGRAPHY
GASTROINTESTINAL TRACT
GLANDS
GROWTH
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIVER
MAMMALS
MATERIALS
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICE
MUSCLES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHENOTYPE
PROTEINS
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
SKELETON
SPLEEN
TISSUE DISTRIBUTION
TISSUES
TRACER TECHNIQUES
TUMOR CELLS
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES