Effects of nitrosocarbaryl on BALB/3T3 cells
Journal Article
·
· Cancer Res.; (United States)
OSTI ID:7286118
Carbaryl(N-methyl-l-naphthylcarbamate) and its nitrosated product, N-nitrosocarbaryl, were tested for their effects on BALB/3T3 (clone A31) cells in culture. Nitrosocarbaryl, but not carbaryl, caused transformation of the BALB/3T3 fibroblasts, but neither chemical induced the complete expression of endogenous murine leukemia virus. Transformed cells differed from the parental control cells by loss of contact inhibition, change in morphology, growth in soft agar, growth to higher saturation densities, and tumorigenicity in normal newborn and irradiated weanling mice and athymic (nude) mice. Transformed clones were found to be negative for expression of RNA tumor virus antigens, viral reverse transcriptase, and infectious virus. Thus, it appears that nitrosocarbaryl can transform BALB/3T3 cells to tumorigenic cells with altered biological properties but without complete activation of RNA tumor viruses in the transformed cells. Expression of viral antigen in the transformed cells was inducible by iododeoxyuridine, indicating that the endogenous viral genome was retained in an unexpressed state. (auth)
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 7286118
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 35; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:5244205
Related Subjects
550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CARCINOGENESIS
CARCINOGENS
CELL CULTURES
CLONE CELLS
CONNECTIVE TISSUE CELLS
DISEASES
FIBROBLASTS
GENETIC EFFECTS
HEMIC DISEASES
LEUKEMIA
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PATHOGENESIS
RODENTS
SOMATIC CELLS
VERTEBRATES
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CARCINOGENESIS
CARCINOGENS
CELL CULTURES
CLONE CELLS
CONNECTIVE TISSUE CELLS
DISEASES
FIBROBLASTS
GENETIC EFFECTS
HEMIC DISEASES
LEUKEMIA
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PATHOGENESIS
RODENTS
SOMATIC CELLS
VERTEBRATES
VIRUSES