Phosphatidylinositol from alcoholic rats is uniquely able to render membranes tolerant to disordering by ethanol

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5217080

Ellingson, J S; Taraschi, T F; Rubin, E

Rat liver microsomal membranes from rats chronically fed ethanol are resistant (tolerant) to membrane disordering by 50-100 mM ethanol. To identify the molecular basis of tolerance, the authors quantitatively separated microsomal phospholipids (PL's) extracted from control and ethanol-fed rats by preparative HPLC, and examined, by electron spin resonance, the structural properties of multilamellar vesicles (MLV's) prepared by recombining control and alcoholic PL's. MLV's made from alcoholic PL's (mixed in same molar ratios as in microsomes) were tolerant to disordering by ethanol, whereas control MLV's were not. If alcoholic phosphatidylcholine (66.5%), phosphatidylethanolamine (21%) or phosphatidylserine (4.0%) replaced their respective PL in control MLV's, the membranes were not tolerant. In contrast, when 8.5% alcoholic phosphatidylinositol (PI) replaced control PI, the MLV's were tolerant. Alcoholic rat PI (8.5%) also conferred tolerance to MLV's containing 91.5% bovine PL's. The authors conclude that the acquisition of membrane tolerance in alcoholic liver microsomes is related to changes in PI.

• Research Organization: Hahnemann Univ. School of Medicine, Philadelphia, PA
• OSTI ID: 5217080
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
CHRONIC EXPOSURE
DIGESTIVE SYSTEM
ELECTRON SPIN RESONANCE
ESTERS
ETHANOL
GLANDS
HYDROXY COMPOUNDS
KINETICS
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAGNETIC RESONANCE
MAMMALS
MEMBRANES
MICROSOMES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHOLIPIDS
RATS
REACTION KINETICS
RESONANCE
RODENTS
SEPARATION PROCESSES
TOLERANCE
VERTEBRATES