New models to identify the molecular mechanisms responsible for membrane tolerance to ethanol

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6015642

Ellingson, J S; Taraschi, T F; Rubin, E

Liver microsomes from rats chronically fed ethanol were resistant to disordering by 50-100 mM ethanol, when studied by ESR techniques. They have developed systems, in which the loss or reacquisition of membrane tolerance to ethanol occurs in a rapid and reproducible manner over a 4 or 2 day period, respectively. This tolerance can be measured in multilamellar vesicles (MLV's) made by recombining the HPLC-separated microsomal phospholipids. Although 5 weeks of ethanol administration were required to develop tolerance, it was concurrently lost in both the microsomes and MLV's after only 4 days of withdrawal. If the 4-day withdrawn rats were refed ethanol, membrane tolerance was partially observed on the 14th day of refeeding, and completely reacquired on the 15th day. Using recombined MLV's they found that microsomal phosphatidylinositol (PI) from 15-day refed rat microsomes could confer tolerance, whereas PI from 13-day and 14-day microsomes could not. The ability of PI to confer membrane tolerance to MLV's was temporally correlated with the acquisition of tolerance in the intact microsomes, strongly indicating that development of membrane tolerance to ethanol in liver microsomes is related to molecular changes in PI.

• Research Organization: Jefferson Medical College, Philadelphia, PA
• OSTI ID: 6015642
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MODELS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHRONIC EXPOSURE
DIGESTIVE SYSTEM
ELECTRON SPIN RESONANCE
ESTERS
ETHANOL
FUNCTIONS
GLANDS
HYDROXY COMPOUNDS
INGESTION
INTAKE
LIPIDS
LIVER
MAGNETIC RESONANCE
MAMMALS
MEMBRANES
MICROSOMES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHOLIPIDS
RATS
RESONANCE
RODENTS
TOLERANCE
VERTEBRATES