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Specific phospholipids in membranes from ethanol-fed rats are responsible for tolerance to disordering by ethanol

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6015646
; ;
The addition of ethanol in vitro (50-100 mM) to liver microsomal and mitochondrial membranes from normal rats causes significant molecular disordering of the membrane bilayer when examined by electron spin resonance (ESR). By contrast, liver microsomes and mitochondria from rats chronically consuming ethanol are resistant to disordering by 100 mM ethanol. They have recently reported that phosphatidylinositol (PI), which constitutes only 8.5% of the microsomal phospholipids, confers membrane tolerance to the microsomal membrane. The ability of microsomal PI from ethanol-fed rats to confer tolerance to other membranes was demonstrated in lipid vesicles composed of bovine brain and liver phospholipids. Using reconstituted phospholipid vesicles and ESR, cardiolipin has been identified as the promoter of membrane tolerance in liver mitochondria isolated from ethanol-fed rats. PI and cardiolipin, although chemically dissimilar, share the common properties of being negatively charged at physiological pH and constituting only 8-10% of the total phospholipid of their respective membranes.
Research Organization:
Jefferson Medical College, Philadelphia, PA
OSTI ID:
6015646
Report Number(s):
CONF-870644-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
BIOLOGICAL FUNCTIONS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
DIGESTIVE SYSTEM
ELECTRON SPIN RESONANCE
ESTERS
ETHANOL
FUNCTIONS
GLANDS
HYDROXY COMPOUNDS
LIPIDS
LIVER
MAGNETIC RESONANCE
MAMMALS
MEMBRANES
MICROSOMES
MITOCHONDRIA
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHOLIPIDS
RATS
RESONANCE
RODENTS
TOLERANCE
TOXICITY
VERTEBRATES