Plasma zinc status and membrane lipid composition in genetically diabetic mice (db/db)
Sex and age matched diabetic C57BL/Ks-db+/db+ mice (db/db) were sacrificed at eight weeks of age. Plasma samples were collected and zinc levels determined. Livers were excised and mitochondrial and microsomal membranes prepared. Aliquots of membrane fractions were subjected to lipid extraction and cholesterol (Cl), phospholipid (PL) and fatty acid analysis (FA) performed. Plasma zinc levels in db/db mice were elevated 25% compared to m/m controls (148.8+/-8.1 ..mu..g/dl vs. 118.9+/-14.9 ..mu..g/dl). Cholesterol and PL levels remained unchanged in both mitochondrial and microsomal membranes. Analysis of PL composition from db/db mitochondria by two dimensional thin layer chromatography revealed no change in the percentage of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) but a 40% decrease in cardiolipin. Slight increases were observed in the percentage of phosphatidylserine and phosphatidylinositol (PS+PI) in microsomes isolated from db/db mice. Fatty acid analysis of microsomal PC and PE showed a decrease of 28% in the 18:1/18:0 ratio as well as a 21% decrease in the ratio of 20:4/18:2 in db/db animals. Analysis of succinate dehydrogenase (mitochondrial) and glucose-6-phosphatase (microsomal) revealed significant decreases in activity in livers of db/db mice. The altered zinc metabolism as well as the changes in membrane lipid composition suggest that this may be a model to study the role of zinc in membrane structure.
- Research Organization:
- Pennsylvania College of Podiatric Medicine, Philadelphia
- OSTI ID:
- 6846528
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Sacchavamyces cerevisiae
Effect of polyunsaturated fatty acids and phospholipids on ( sup 3 H)-vitamin E incorporation into pulmonary artery endothelial cell membranes
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL COMPOSITION
CHOLESTEROL
CHROMATOGRAPHY
DIABETES MELLITUS
DISEASES
DISTRIBUTION
ELEMENTS
ENDOCRINE DISEASES
ENZYMES
HYDROXY COMPOUNDS
LIPIDS
MAMMALS
MATERIALS
MEMBRANES
METABOLIC DISEASES
METABOLISM
METALS
MICE
MICROSOMES
MITOCHONDRIA
ORGANIC COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
PATHOGENESIS
RODENTS
SEPARATION PROCESSES
STEROIDS
STEROLS
THIN-LAYER CHROMATOGRAPHY
TISSUE DISTRIBUTION
VERTEBRATES
ZINC