Physical properties, lipid composition and enzyme activities of hepatic subcellular membranes from chick embryo after ethanol treatment

Journal Article · Tue Dec 31 23:00:00 EST 1991 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(92)90308-C· OSTI ID:6952272

Sanchez-Amate, M C; Marco, C; Segovia, J L ^[1]

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1. Univ. of Granada (Spain)

Exposure of chick embryos to ethanol resulted in significant alterations to the lipid composition of various different hepatic subcellular membranes. A marked decrease in cholesterol levels and an increase in the phospholipid content of microsomes and mitochondria was observed. Ethanol also affected the fatty acid profiles, mainly by decreasing the percentage of oleic acid in phosphatidylcholine and phosphatidylethanolamine in the mitochondria and phosphatidylethanolamine in the microsomes. In spite of these changes ethanol only induced alterations in the fluidity of the mitochondrial membranes, which showed a more rigid core, in contrast to the phospholipid-head region, which was not affected. In accordance with the changes observed in the physical state of the membrane, the enzymes involved in the microsomal electron-transport systems were not modified by ethanol, while cytochrome oxidase activity decreased by 50% compared to the activity in the mitochondria from control chick embryos.

• OSTI ID: 6952272
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 51:21; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
BIRDS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL COMPOSITION
CHICKENS
CYTOCHROME OXIDASE
DIGESTIVE SYSTEM
EMBRYOS
ENZYME ACTIVITY
ENZYMES
ETHANOL
FOWL
GLANDS
HAEM DEHYDROGENASES
HYDROXY COMPOUNDS
LIPIDS
LIVER
MEMBRANES
METABOLISM
MICROSOMES
MITOCHONDRIA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHYSICAL PROPERTIES
PROTEINS
RIBOSOMES
SENSITIVITY
VERTEBRATES