Endogenous leukotriene D/sub 4/ formation during anaphylactic shock in the guinea pig

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.16.5903· OSTI ID:5206264

Keppler, A; Oerning, L; Bernstroem, K; Hammarstroem, S

Experiments on the metabolism and excretion of i.v. administered selectively labeled (/sup 3/H/sub 8/)leukotriene C/sub 4/ in bile duct-cannulated guinea pigs indicated predominantly biliary excretion of tritium. The major leukotriene metabolite in bile was identified as leukotriene D/sub 4/. By monitoring leukotriene excretion radioimmunochromatographically, it was shown that guinea pigs suffering from anaphylactic shock produce leukotriene D/sub 4/ endogenously. Immunological challenge of animals sensitized to ovalbumin was accompanied by an increase of biliary leukotriene D/sub 4/ concentrations from 10 +/- 1 to 86 +/- 10 nM. When considering that bile flow was decreased to about half after challenge, the excretion rate of leukotriene D/sub 4/ in bile increased from 0.88 +/- 0.16 before 3.18 +/- 0.38 pmol x min/sup -1/ x kg/sup -1/ after challenge. It is concluded that systemic anaphylaxis in the guinea pig is associated with endogenous generation of leukotriene C/sub 4/.

• Research Organization: Karolinska Institutet, Stockholm (Sweden)
• OSTI ID: 5206264
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:16; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
ANIMALS
BILE
BIOLOGICAL MATERIALS
BODY FLUIDS
CHROMATOGRAPHY
CLEARANCE
DRUGS
EXCRETION
GUINEA PIGS
HEMATOLOGIC AGENTS
IMMUNOASSAY
IMMUNOLOGY
INJECTION
INTAKE
INTRAVENOUS INJECTION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOCHROMATOGRAPHY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES