STUDIES ON WHOLE-BODY X IRRADIATION EFFECTS ON ANAPHYLACTIC HYPERSENSITIVITY IN GUINEA PIGS

Journal Article · Sun Jan 01 04:00:00 EST 1961 · Acta Allergologica (Denmark) Changed to Allergy (Copenhagen)

OSTI ID:4751465

Vlahovic, S; Stankovic, V

Guinea pigs were sensitized with and then challenged with horse serum 14 days later. The anaphylactic reactions of this group were taken as control and compared with the anaphylactic reactions observed in animals sensitized from 30 min to 14 days before irradiation (350 or 400 r), and with the reactions observed in animals sensitized from 30 min to 14 days after the exposure to the same doses of x rays. The reactions in animals sensitized after irradiation were much less than those observed in controls; no lethal response was observed. However, the anaphylactic response in animals sensitized before irradiation showed a higher percentage of fatal reaction than in the unirradiated group. The lethal anaphylactic response depended on how long before irradiation sensitization was performed. Eighty percent of guinea pigs sensitized from 1 to 14 days before irradiation gave a fatal reaction on subsequent challenge. Animals sensitized 30 min, 8 hr, or 12 hr before irradiation showed the same mortality rate as the control unirradiated group. In experiments on the passive transfer of anaphylactic hypersensitivity, pooled blood serum obtained from normal or irradiated guinea pigs 14 days after sensitization, viz. 15 days after irradiation, was injected intraperitoneally into normal or irradiated animals. Serum obtained from normal guinea pigs 14 days after sensitization was injected intraperitoneally into normal or irradiated animals (5 days after exposure to 400 or 450 r). All passively sensitized animals showed an anaphylactic reaction after challenge (24 hr after receiving the sensitizing serum), and no effect of irradiation was detectable. In the 2nd experiment, serum was obtained from guinea pigs sensitized 24 hr after irradiation with 300 r. The normal recipients manifested an appreciably weaker anaphylactic response to challenge than after receiving serum from normal sensitized animals. Thus, the blood of irradiated sensitized animals contained fewer circulating antibodies. (H.H.D.)

• Research Organization: Inst. Rudjer Boskovic, Zagreb
• NSA Number: NSA-17-005783
• OSTI ID: 4751465
• Journal Information: Acta Allergologica (Denmark) Changed to Allergy (Copenhagen), Journal Name: Acta Allergologica (Denmark) Changed to Allergy (Copenhagen) Vol. Vol: 16; ISSN ACALA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANTIBODIES
BIOLOGY AND MEDICINE
BLOOD SERUM
BODY
DISEASES
GUINEA PIGS
IMMUNITY
LIFETIME
QUANTITY RATIO
RADIATION DOSES
RADIATION EFFECTS
X RADIATION