A sequence homologous to kappa-deleting element is located 5 prime to the human J sub K locus

Journal Article · Wed Jan 25 04:00:00 EST 1989 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/17.2.809· OSTI ID:5190838

Kennedy, M A; Morris, C M; Fitzgerald, P H ^[1]

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1. Christchurch Hospital (New Zealand)

The human kappa deleting element (Kde) mediates loss of CK and JK genes in B cells. A probe for Kde detects two genomic sequences on Southern blots. The Kde is located 24kb 3{prime} to CK, but the position of the homologous sequence is unknown. The authors in situ hybridized m141-2 to metaphase cells of JC11, a B-cell line bearing a t(2;14)(p11;q32) in which the chromosome 2 breakpoint is within JK or the VK-JK intron. Three peaks of labelled sites were obtained. Southern analysis of BamH1 digested DNA showed that Kde (14kb) and the homologous sequence (3kb) were both intact. Kde accounts for hybridization to 14q+ and the 2p- signal presumably derives from the related sequence. This locates the sequence homologous to Kde upstream from JK, possibly within the VK cluster, and may reflect transposition or some other duplicative event as proposed for the evolution of other regions of the kappa locus.

• OSTI ID: 5190838
• Journal Information: Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 17:2; ISSN 0305-1048; ISSN NARHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
CHROMOSOMES
DNA
DNA SEQUENCING
DNA-ASE
ENZYMES
ESTERASES
GENES
HETEROCHROMOSOMES
HYBRIDIZATION
HYDROLASES
MAMMALS
MAN
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES