Molecular analysis of TCRB and ABL in a t(7; 9)-containing cell line (SUP-T3) from a human T-cell leukemia

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.1.251· OSTI ID:5520169

Westbrook, C A; Rubin, C M; Le Beau, M M; Kaminer, L S; Smith, S D; Rowley, J D; Diaz, M O

A translocation between chromosomes 7 and 9, t(7;9), has been described in cell lines derived from the malignant cells of children with acute T-cell lymphoblastic leukemia or lymphoma. The cytogenetic analysis of one such cell line, SUP-T3, demonstrates that the breakpoints on chromosomes 7 and 9 lie within bands q36 and q34, respectively, corresponding to the location of the gene encoding the ..beta.. chain of the T-cell receptor, TCRB, and the gene homologous to the transforming gene of the Abelson murine leukemia virus, ABL. The authors investigated the role of these genes in the t(7;9). In situ chromosomal hybridization of TCRB and ABL probes to metaphase cells from SUP-T3 demonstrated that ABL is translocated from chromosome 9 to 7 and that all or part of TCRB is translocated from chromosome 7 to 9. Southern blot analysis revealed that both TCRB alleles were rearranged; however, it could not be determined whether the translocation breakpoint lies within this gene. Pulsed-field gel electrophoresis and Southern blot analysis were used to examine more than 500 kilobases on the ABL locus; they concluded that there are no rearrangements within 250 kb in either direction of the sequences homologous to v-abl. These results indicate that, in SUP-T3, the breakpoint on chromosome 9 lies proximal to ABL and the break results in no apparent alteration of the ABL protein. They therefore hypothesize that another gene of chromosome 9, at band q34, plays a role in this translocation. This study also demonstrates that pulsed-field gel electrophoresis is a powerful new tool for the analysis of human chromosomal translocations.

• Research Organization: Univ. of Chicago, IL
• OSTI ID: 5520169
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:1; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE GROUPS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CARCINOGENESIS
CELL TRANSFORMATIONS
CHILDREN
CHROMOSOMAL ABERRATIONS
CYTOLOGICAL TECHNIQUES
DAYS LIVING RADIOISOTOPES
DISEASES
GENES
GENETIC MAPPING
GENETICS
HEMIC DISEASES
HYBRIDIZATION
ISOTOPES
KARYOTYPE
LABELLED COMPOUNDS
LEUKEMIA
LIGHT NUCLEI
MAPPING
MEMBRANE PROTEINS
MUTATIONS
NEOPLASMS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PATHOGENESIS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECEPTORS
TRITIUM COMPOUNDS
TUMOR CELLS