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Cloning of the chromosome translocation breakpoint junction of the t(14; 19) in chronic lymphocytic leukemia

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
The authors' laboratory has reported that t(14;19)(q32;q13.1) is a recurring translocation in the neoplastic cells of patients with chronic lymphocytic leukemia. In the present study, they have analyzed the leukemic cells from one such patient with probes from the immunoglobulin heavy-chain locus, which is present on band q32 of chromosome 14. Using a probe for the ..cap alpha.. constant-region gene segments, they detected a rearranged band by Southern blot analysis. This rearranged band was cloned and mapped. A subclone free of repetitive sequences was shown to be from chromosome 19 by analysis of human-mouse somatic cell hybrids, confirming that the rearranged band contains the translocation breakpoint junction. This probe may be used to identify a gene on chromosome 19 adjacent to the breakpoint that can contribute to the malignant development of B lymphocytes.
Research Organization:
Univ. of Chicago, IL (USA)
DOE Contract Number:
FG02-86ER60408
OSTI ID:
6464147
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:24; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CELL TRANSFORMATIONS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CLONING
DAYS LIVING RADIOISOTOPES
DISEASES
DNA HYBRIDIZATION
DNA-CLONING
ELECTROPHORESIS
GENETIC MAPPING
GENETICS
GLOBULINS
HEMIC DISEASES
HETEROCHROMOSOMES
HUMAN CHROMOSOME 19
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
IMMUNOGLOBULINS
ISOTOPES
LEUKEMIA
LIGHT NUCLEI
MAPPING
MUTATIONS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PATIENTS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
TUMOR CELLS