Defining the breakpoint of a multigene deletion in the immunoglobulin heavy chain gene cluster
- Univ. of Toronto, Ontario (Canada)
The constant region of the human immunoglobulin heavy chain (IGHC) is encoded by a cluster of genes near the telomere of chromosome 14q. Deletions and duplications of single or multiple genes in the cluster have been identified, but little information about the breakpoint junctions has been available, in part due to the high degree of sequence similarity between the genes in this region. We report an intensive study of a homozygous deletion, using Southern hybridization and polymerase chain reaction techniques. We found that the deleted DNA includes the functional epsilon gene, and that the breakpoints are located within a 2 kilobase Bam HI/Sac I region of both the IGHEP1 and IGHE genes. These results revise a previous conclusion regarding the deleted region. Definition of breakpoints occurring within this cluster may shed light on recombination mechanisms. 16 refs., 4 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 75603
- Journal Information:
- Immunogenetics, Vol. 41, Issue 2-3; Other Information: PBD: 11 Jan 1995
- Country of Publication:
- United States
- Language:
- English
Similar Records
Variant translocation of the bcl-2 gene to immunoglobulin. lambda. light chain gene in chronic lymphocytic leukemia
Breakpoint of an inversion of chromosome 14 in a T-cell leukemia: sequences downstream of the immunoglobulin heavy chain locus are implicated in tumorigenesis