Defining the breakpoint of a multigene deletion in the immunoglobulin heavy chain gene cluster
- Univ. of Toronto, Ontario (Canada)
The constant region of the human immunoglobulin heavy chain (IGHC) is encoded by a cluster of genes near the telomere of chromosome 14q. Deletions and duplications of single or multiple genes in the cluster have been identified, but little information about the breakpoint junctions has been available, in part due to the high degree of sequence similarity between the genes in this region. We report an intensive study of a homozygous deletion, using Southern hybridization and polymerase chain reaction techniques. We found that the deleted DNA includes the functional epsilon gene, and that the breakpoints are located within a 2 kilobase Bam HI/Sac I region of both the IGHEP1 and IGHE genes. These results revise a previous conclusion regarding the deleted region. Definition of breakpoints occurring within this cluster may shed light on recombination mechanisms. 16 refs., 4 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 75603
- Journal Information:
- Immunogenetics, Journal Name: Immunogenetics Journal Issue: 2-3 Vol. 41; ISSN 0093-7711; ISSN IMNGBK
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mapping of human immunoglobulin heavy chain variable gene segments outside the major IGH locus
Molecular basis of human growth hormone gene deletions
Variant translocation of the bcl-2 gene to immunoglobulin. lambda. light chain gene in chronic lymphocytic leukemia
Journal Article
·
Thu Sep 01 00:00:00 EDT 1994
· Genomics
·
OSTI ID:186063
Molecular basis of human growth hormone gene deletions
Journal Article
·
Mon Aug 01 00:00:00 EDT 1988
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5404636
Variant translocation of the bcl-2 gene to immunoglobulin. lambda. light chain gene in chronic lymphocytic leukemia
Journal Article
·
Fri Mar 31 23:00:00 EST 1989
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5352534