Radioimmunoassay and liquid-chromatographic analysis for free cortisol in urine compared with isotope dilution-mass spectrometry

Journal Article · Sat May 01 04:00:00 EDT 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5176777

Lantto, O

Three different routine methods for analysis for urinary cortisol with those by a highly specific reference method based on isotope dilution-mass spectrometry (I) were compared. A ''high-performance'' liquid-chromatographic method (II) gave the most comparable results (regression coefficient 0.86, intercept 9 nmol/L). For some urines much lower values were obtained by I than by II. Two radioimmunoassay (III) methods, one involving direct assay and one involving extraction, gave less-accurate results (regression coefficients of 1.87 and 1.52 and intercepts of 86 and 12 nmol/L, respectively), although values obtained by III and by I correlated well (r = 0.95-0.99), indicating a relation between the free cortisol and the compounds interfering in III. The apparent accuracy for the extraction method was improved by using as calibration standards urine samples previously assayed by I (regression coefficient 0.90, intercept 6 nmol/L). All four methods investigated showed a statistically significant sex-related difference in 24-h urinary cortisol excretion; evidently such a finding should be a prerequisite in any such method proposed for routine use.

• Research Organization: Huddinge Univ. Hospital, Sweden
• OSTI ID: 5176777
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:5; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CORTICOSTEROIDS
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
MATERIALS
MEASURING METHODS
ORGANIC COMPOUNDS
PATIENTS
PREGNANES
RADIOASSAY
RADIOIMMUNOASSAY
SEPARATION PROCESSES
SEX DEPENDENCE
SPECTROSCOPY
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
URINE
WASTES