Comparison of radioimmunoassay with thin-layer chromatographic and gas-- liquid chromatographic methods of barbiturate detection in human urine

Journal Article · Thu May 01 04:00:00 EDT 1975 · Clin. Chem., v. 21, no. 6, pp. 672-675

OSTI ID:4163332

Roerig, D L; Lewand, D L; Mueller, M A; Wang, R I.H.

A radioimmunoassay (I) for barbiturates was compared with thin-layer chromatographic (II) and gas--liquid chromatographic (III) methods for barbiturate detection in human urine. Timed urine samples were obtained from volunteers who had ingested 100 mg of a barbiturate. I detected barbiturate in all urines tested up to 76 h after the dose, and III in all up to 52 h and in 90 percent up to 76 h. II detected barbiturates in 90 percent of all urine samples for only 30 h, after which its reliability declined. Glutethimide interfered with radioimmunoassay of barbiturate, producing false positives. I is sensitive, reliable, and fast, and lends itself to screening large numbers of urine samples for barbiturates. For routine urine surveillance, however, we found I to be less useful than II, which is still the method of choice. I has, however, proved to be an excellent method for confirming results of II. (auth)

• Research Organization: Veterans Administration Center, Milwaukee
• NSA Number: NSA-33-003401
• OSTI ID: 4163332
• Journal Information: Clin. Chem., v. 21, no. 6, pp. 672-675, Journal Name: Clin. Chem., v. 21, no. 6, pp. 672-675; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

*BARBITURATES-- RADIOIMMUNOASSAY
550601* --Biomedical Sciences
Basic Studies--Medicine-- Unsealed Radionuclides in Diagnostics
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
IODINE 125
LABELLED COMPOUNDS
MAN
N48830* --Life Sciences--New Tracer Techniques--Other
THIN-LAYER CHROMATOGRAPHY
URINE