Radioimmunoassay of methaqualone in human urine compared with chromatographic methods. [/sup 125/I tracer technique]

Journal Article · Fri Sep 01 04:00:00 EDT 1978 · Clin. Chem.; (United States)

OSTI ID:6340474

Mule, S J; Kogan, M; Jukofsky, D

The /sup 125/I-radioimmunoassay for methaqualone in human urine was evaluated by a comparison with newly modified gas-liquid chromatographic and thin-layer chromatographic methods. The statistically significant sensitivity value for the radioimmunoassay was at 2 ..mu..g of methaqualone per liter of urine. The coefficient of variation was 2.88 -+ 0.16% intraassay. There was cross-reactivity only with metabolites of methaqualone, 4'-hydroxymethaqualone being twice as sensitively measured as methaqualone. There was complete agreement between results by radioimmunoassay and by gas-liquid chromatography in 96.7% of the samples analyzed. Only 1.2% of the radioimmunoassay values were false positives, and 2.1% false negatives (phi = 0.8917, P < 0.001). Comparisons between the thin-layer chromatographic data and the gas--liquid chromatographic or radioimmunoassay data showed less agreement because of the 50- to 200-fold higher sensitivity of the latter techniques. Gas--liquid chromatography therefore appears to represent the best reference method for the evaluation of the radioimmunoassay, which appears to be a very sensitive and reliable technique for detecting methaqualone and its metabolites in human urine.

• Research Organization: DSAS Testing and Research Lab., Brooklyn, NY
• OSTI ID: 6340474
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 24:9; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HYPNOTICS AND SEDATIVES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATHEMATICS
NUCLEI
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
STATISTICS
TRACER TECHNIQUES
URINE
WASTES